Skip to main content
Addgene

pLenti-puro
(Plasmid #39481)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39481 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart
Cloning Grade DNA 39481-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105
Add to Cart

Backbone

  • Vector backbone
    pLenti4/TO/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Removed the Gateway elements between the 2 EcoRV sites and replaced the Zeocin resistance gene with a puromycin resistance gene
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter CMV
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • V5 and 6XHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    See Depositor Comments below.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer V5-rev and BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note this plasmid appears to be unstable during growth in bacteria. We recommend screening 3-5 smaller colonies after streaking from the original stab. It may be necessary to cut the plasmid with a single-cutting enzyme, gel purify the high molecular weight band, and religate to restore the plasmid.

Information for Cloning Grade DNA (Catalog # 39481-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481 ; http://n2t.net/addgene:39481 ; RRID:Addgene_39481)

  • For your References section:

    ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401

Ready-to-use antibodies

Looking for antibodies related to this plasmid? Check out these options:

Learn More
Related items:
Commonly requested with: