pGEX-p53 R175H
(Plasmid #39480)

• Depositing Lab: Ie-Ming Shih
• Publication: Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.

Backbone

• Vector backbone: pGEX-6p-1
• Backbone manufacturer: Amersham
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TP53
• Species: H. sapiens (human)
• Mutation: P72R and R175H
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI to SalI (destroyed during cloning)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the p53 insert in this plasmid contains a known P72R variant when compared to GenBank ID NP_000537.3