pLenti-puro-ARID1A
(Plasmid #39478)

• Purpose: Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector
• Depositing Lab: Ie-Ming Shih
• Publication: Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.

Backbone

• Vector backbone: pLenti-puro
• Total vector size (bp): 13944
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARID1A
• Species: H. sapiens (human)
• Insert Size (bp): 6854
• GenBank ID: NM_006015.4
• Entrez Gene: ARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • His (C terminal on backbone)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI to XhaI (destroyed during cloning)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 39478
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry