pLenti-puro-LacZ
(Plasmid #39477)

• Depositing Lab: Ie-Ming Shih
• Publication: Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.

Backbone

• Vector backbone: pLenti-puro
• Total vector size (bp): 10242
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LacZ
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pLenti6/V5-GW/LacZ, Invitrogen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry