pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA
(Plasmid #39455)

• Depositing Lab: Hongjun Song
• Publication: Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 2011 Apr 14.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5321
• Total vector size (bp): 7523
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HA-hTet1CDmu
• Species: H. sapiens (human)
• Insert Size (bp): 2202
• Mutation: aa1418–2136 with mutations H1672 to Y, 1674D to A
• Entrez Gene: TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
• Promoter: EF1a
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: EF1A-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry