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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39454 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5321
- Total vector size (bp) 7523
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-hTet1CD
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SpeciesH. sapiens (human)
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Insert Size (bp)2202
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Mutationaa1418–2136
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Entrez GeneTET1 (a.k.a. CXXC6, LCX, bA119F7.1)
- Promoter EF1a
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer EF1A-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA was a gift from Hongjun Song (Addgene plasmid # 39454 ; http://n2t.net/addgene:39454 ; RRID:Addgene_39454) -
For your References section:
Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain. Guo JU, Su Y, Zhong C, Ming GL, Song H. Cell. 2011 Apr 29;145(3):423-34. Epub 2011 Apr 14. 10.1016/j.cell.2011.03.022 PubMed 21496894