TAL2071
(Plasmid #39441)

• Depositing Lab: Keith Joung
• Publication: Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170.

Backbone

• Vector backbone: JDS78
• Backbone manufacturer: Joung lab (Addgene Plasmid # 32290)
• Backbone size w/o insert (bp): 6447
• Vector type: Mammalian Expression ; T7

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP-TALEN-42-Right
• Species: H. sapiens (human)
• Insert Size (bp): 1742
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3X Flag (N terminal on backbone)
  • WT FOKI (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Target binding site: TGTGGCGGATCTTGAAGTT

To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.

It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.4kb and 5.8kb