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Addgene

TAL2011
(Plasmid #39369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39369 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JDS71
  • Backbone manufacturer
    Joung lab (Addgene Plasmid # 32287)
  • Backbone size w/o insert (bp) 6447
  • Vector type
    Mammalian Expression ; T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-TALEN-06-Right
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    926
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3X Flag (N terminal on backbone)
    • WT FOKI (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target binding site: TGCAGATGAAC

To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.

It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL2011 was a gift from Keith Joung (Addgene plasmid # 39369 ; http://n2t.net/addgene:39369 ; RRID:Addgene_39369)

  • For your References section:

    FLASH assembly of TALENs for high-throughput genome editing. Reyon D, Tsai SQ, Khayter C, Foden JA, Sander JD, Joung JK. Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170. 10.1038/nbt.2170 PubMed 22484455
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