pFA6a-kanMX6-Purg1-3HA
(Plasmid #39351)

• Depositing Lab: Jurg Bahler
• Publication: Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Backbone

• Vector backbone: pFA6a-kanMX6-P3nmt1
• Backbone manufacturer: Addgene plasmid # 39280
• Backbone size w/o insert (bp): 2300
• Modifications to backbone: nmt1 promoter has been replaced with urg1 promoter.
• Vector type: Yeast genomic targeting
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: urg1 promoter
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2400
• Entrez Gene: urg1 (a.k.a. SPAC1002.19)
• Entrez Gene: urg1 (a.k.a. SPAC1002.19)
• Tags / Fusion Proteins:
  • 3xHA (C terminal on insert)
  • kanMX6 module - reverse orientation (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: Sp6_primer
• 3′ sequencing primer: T7_promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry