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Addgene

pFA6a-natMX6-Purg1
(Plasmid #39350)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39350 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-natMX6-P3nmt1
  • Backbone manufacturer
    Addgene plasmid # 39280
  • Backbone size w/o insert (bp) 2300
  • Modifications to backbone
    nmt1 promoter has been replaced with urg1 promoter. kanMX6 replaced with natMX6
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    nourseothricin (NAT)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    urg1 promoter
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1900
  • Entrez Gene
    urg1 (a.k.a. SPAC1002.19)
  • Entrez Gene
    urg1 (a.k.a. SPAC1002.19)
  • Tag / Fusion Protein
    • natMX6 module - reverse orientation (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer Sp6_primer
  • 3′ sequencing primer T7_promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-natMX6-Purg1 was a gift from Jurg Bahler (Addgene plasmid # 39350 ; http://n2t.net/addgene:39350 ; RRID:Addgene_39350)

  • For your References section:

    urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. Watt S, Mata J, Lopez-Maury L, Marguerat S, Burns G, Bahler J. PLoS One. 2008 Jan 16;3(1):e1428. 10.1371/journal.pone.0001428 PubMed 18197241
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