Skip to main content
Addgene

anti-Desmin_D7-pBIOCAM5
(Plasmid #39346)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBIOCAM5-3F
  • Backbone manufacturer
    McCafferty lab unpublished
  • Total vector size (bp) 7004
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    scFv-Fc-fusion specific for Desmin (clone D7)
  • Species
    H. sapiens (human)
  • Entrez Gene
    DES (a.k.a. CDCD3, CSM1, CSM2, LGMD1D, LGMD1E, LGMD2R)
  • Tags / Fusion Proteins
    • human Fc (C terminal on backbone)
    • 6xHis (C terminal on backbone)
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-Desmin_D7-pBIOCAM5 was a gift from John McCafferty (Addgene plasmid # 39346 ; http://n2t.net/addgene:39346 ; RRID:Addgene_39346)

  • For your References section:

    Application of phage display to high throughput antibody generation and characterization. Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SDj, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J. Genome Biol. 2007;8(11):R254. 10.1186/gb-2007-8-11-r254 PubMed 18047641

Ready-to-use antibodies

Looking for antibodies expressed from this plasmid? Check out this option:

Learn More
Related items:
Commonly requested with: