pAAV-Ef1a-DIO-VMAT2-WPRE-pA
(Plasmid #39339)

• Depositing Lab: Bernardo Sabatini
• Publication: Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi: 10.1038/nature11466. Epub 2012 Oct 3.

Backbone

• Vector backbone: pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA
• Backbone manufacturer: K. Deisseroth Lab
• Backbone size w/o insert (bp): 5603
• Total vector size (bp): 6314
• Modifications to backbone: None
• Vector type: AAV ; r

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Slc18a2
• Alt name: VMAT2 (vesicular monoamine transporter 2)
• Alt name: SVAT (synaptic vesicle amine transporter)
• Species: M. musculus (mouse)
• Insert Size (bp): 1584
• Mutation: Myc tag inserted in the first lumenal loop between Glycines 98 and 99
• GenBank ID: NM_172523.3
• Entrez Gene: Slc18a2 (a.k.a. 1110037L13Rik, 9330105E13, Vmat2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Asc1 (not destroyed)
• 3′ cloning site: Nhe1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene was custom synthesized and cloned in the rAAV backbone as indicated

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry