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pET21a(+)-HpDsbG C162A
(Plasmid #39330)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39330 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6148
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    oxidoreductase
  • Species
    Helicobacter pylori
  • Insert Size (bp)
    705
  • Mutation
    changed Cysteine 162 to Alanine
  • GenBank ID
    AAD07309.1
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct can be expressed in C41(DE3) cells; please see Miroux and Walker, J Mol Biol, 1996.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-HpDsbG C162A was a gift from SeWon Suh (Addgene plasmid # 39330 ; http://n2t.net/addgene:39330 ; RRID:Addgene_39330)

  • For your References section:

    Structural and functional characterization of Helicobacter pylori DsbG. Yoon JY, Kim J, Lee SJ, Kim HS, Im HN, Yoon HJ, Kim KH, Kim SJ, Han BW, Suh SW. FEBS Lett. 2011 Dec 15;585(24):3862-7. Epub 2011 Nov 2. 10.1016/j.febslet.2011.10.042 PubMed 22062156
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