pMSP1E3D1_D73C
(Plasmid #39328)

• Depositing Lab: Stephen Sligar
• Publication: Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Backbone

• Vector backbone: pET 28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: for protein expression use BL21gold(DE3); high oxygenation level
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MSP1E3D1_D73C
• Alt name: membrane scaffold protein
• Alt name: APOA1
• Species: synthetic gene
• Insert Size (bp): 840
• Mutation: cys mutant of MSP1E3D1
• Entrez Gene: APOA1 (a.k.a. AMYLD3, HPALP2, apo(a))
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry