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Addgene

human EB1 in pBluescript II
(Plasmid #39324)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II
  • Backbone size w/o insert (bp) 2961
  • Total vector size (bp) 3765
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    804
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human EB1 in pBluescript II was a gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39324 ; http://n2t.net/addgene:39324 ; RRID:Addgene_39324)
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