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pMJ841
(Plasmid #39318)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39318 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEC-K-MBP
  • Backbone manufacturer
    pET-based custom vector
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 10500
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Low Kan concentration required, use 25ug/mL
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Csn1
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4104
  • Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 to Alanine (H840A); catalytically inactive
  • GenBank ID
    AAK33936.1
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer MBP-forward
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Doudna Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/doudna/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJ841 was a gift from Jennifer Doudna (Addgene plasmid # 39318 ; http://n2t.net/addgene:39318 ; RRID:Addgene_39318)

  • For your References section:

    A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829. Epub 2012 Jun 28. 10.1126/science.1225829 PubMed 22745249
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