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Addgene

human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)
(Plasmid #39306)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5504
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    804
  • Mutation
    E211A, K212A, E213A, R214A, D215A and Y217A
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145) was a gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39306 ; http://n2t.net/addgene:39306 ; RRID:Addgene_39306)
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