human EB1 in pEGFP N1 to express EB1-GFP (JB131)
(Plasmid #39299)

• Depositing Labs: Tim Mitchison, Jennifer Tirnauer
• Publication: Tirnauer lab EB1 plasmids (unpublished)

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5504
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB1
• Alt name: MAPRE1
• Species: H. sapiens (human)
• Insert Size (bp): 804
• Entrez Gene: MAPRE1 (a.k.a. EB1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Makes beautiful comets on tips of microtubules in all cells tested. Clones of Ptk1 cells stably expressing this plasmid were made. Contact me for further details. JST