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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6173
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsCan be propagated in DH5alpha. For protein expression, grow in BL21 pLysS strain. Induce with IPTG about 3 hours and purify on Nickel agarose. For the full expression and purification protocol see Tirnauer et al MBoC 2002 EB1–Microtubule Interactions in Xenopus Egg Extracts: Role of EB1 in Microtubule Stabilization and Mechanisms of Targeting to Microtubules
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEB1
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Alt nameMAPRE1
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SpeciesH. sapiens (human)
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Insert Size (bp)804
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Entrez GeneMAPRE1 (a.k.a. EB1)
- Promoter T7
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Tag
/ Fusion Protein
- 6HIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 promotor primer
- 3′ sequencing primer T7 terminator primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJen-Yu (technician in Barbara Bierer's lab)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sequenced. See Berrueta et al 1999
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human EB1 in PET28a for protein expression was a gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39297 ; http://n2t.net/addgene:39297 ; RRID:Addgene_39297) -
For your References section:
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. Tirnauer JS, Grego S, Salmon ED, Mitchison TJ. Mol Biol Cell. 2002 Oct . 13(10):3614-26. 10.1091/mbc.E02-04-0210 PubMed 12388761