pFA6a-GST-kanMX6
(Plasmid #39293)

• Depositing Labs: Jurg Bahler, John Pringle
• Publication: Bahler et al Yeast. 1998 Jul;14(10):943-51.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 2300
• Vector type: Yeast genomic targeting
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KanR
• Alt name: kanamycin resistance cassette
• Insert Size (bp): 2500
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • yeast ADH1 terminator (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: Sp6_primer
• 3′ sequencing primer: T7_promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry