pFA6a-kanMX6-P81nmt1-GFP
(Plasmid #39291)

• Depositing Labs: Jurg Bahler, John Pringle
• Publication: Bahler et al Yeast. 1998 Jul;14(10):943-51.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 3500
• Vector type: Yeast genomic targeting
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: nmt1 promoter
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2900
• Mutation: -1163 to +6 of nmt1 locus with a 7bp deletion (ATATAAAA) ~100bp from 3' end of promoter
• Entrez Gene: nmt1 (a.k.a. SPCC1223.02)
• Tags / Fusion Proteins:
  • A. gossypii translation elongation factor 1a gene terminator (N terminal on insert)
  • KanR reverse orientation (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
  • GFP (C terminal on insert)
  • TADH1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: Sp6_primer
• 3′ sequencing primer: T7_promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use GFP reverse primer to verify mutation in nmt1 promoter