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Addgene

pATCH1
(Plasmid #39274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKSS
  • Backbone manufacturer
    Peter Kast Lab
  • Backbone size w/o insert (bp) 2443
  • Total vector size (bp) 2863

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Updated MCS for pET vectors with enhanced stop codons
  • Insert Size (bp)
    420
  • GenBank ID
    AF013597

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site DraIII (not destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pET vectors have a leaky UGA stop codon. Plasmid pATCH1 provides a replacement sequence for the inefficient stop signal and can be used to repair both pET vectors and existing pET-based expression constructs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pATCH1 was a gift from Peter Kast (Addgene plasmid # 39274 ; http://n2t.net/addgene:39274 ; RRID:Addgene_39274)

  • For your References section:

    UGA read-through artifacts--when popular gene expression systems need a pATCH. MacBeath G, Kast P. Biotechniques. 1998 May;24(5):789-94. 10.2144/98245st02 PubMed 9591128
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