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pcDNA3.Flag.HERC2-HECT-C4762A.6xHis
(Plasmid #39228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HERC2 HECT Domain
  • Alt name
    HERC2
  • Alt name
    HECT and RLD domain containing E3 ubiquitin protein ligase 2
  • Alt name
    p528
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    C4762A
  • GenBank ID
    NM_004667.5 Uniprot: O95714[4457-4794]
  • Entrez Gene
    HERC2 (a.k.a. D15F37S1, MRT38, SHEP1, jdf2, p528)
  • Promoter T7
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HECT domain active site point mutation (C4762A substitution)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.Flag.HERC2-HECT-C4762A.6xHis was a gift from Aziz Sancar (Addgene plasmid # 39228 ; http://n2t.net/addgene:39228 ; RRID:Addgene_39228)

  • For your References section:

    Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein. Kang TH, Reardon JT, Sancar A. Nucleic Acids Res. 2011 Apr;39(8):3176-87. Epub 2010 Dec 30. 10.1093/nar/gkq1318 PubMed 21193487
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