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Addgene

p2Bac.Flag.p34
(Plasmid #39221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2Bac
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7125
  • Total vector size (bp) 8123
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p34
  • Alt name
    GTF2H3
  • Alt name
    general transcription factor IIH, polypeptide 3, 34kDa
  • Alt name
    BTF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • GenBank ID
    NM_001516.3
  • Entrez Gene
    GTF2H3 (a.k.a. BTF2, P34, TFB4, TFIIH)
  • Promoter Pph
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer polyhedrin_fwd
  • 3′ sequencing primer polyhedrin_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2Bac.Flag.p34 was a gift from Aziz Sancar (Addgene plasmid # 39221 ; http://n2t.net/addgene:39221 ; RRID:Addgene_39221)
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