p2Bac.His.XPD
(Plasmid
#39217)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonep2Bac
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7125
- Total vector size (bp) 9423
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameXPD
-
Alt nameERCC2
-
Alt nameexcision repair cross-complementing rodent repair deficiency, complementation group 2
-
Alt nameRad3
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2300
-
MutationXPD starts at aa4
-
GenBank IDNM_001130867.1 NM_000400.3
-
Entrez GeneERCC2 (a.k.a. COFS2, EM9, TFIIH, TTD, TTD1, XPD)
- Promoter Pph
-
Tag
/ Fusion Protein
- His (HHYHHH) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer polyhedrin_fwd
- 3′ sequencing primer polyhedrin_rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
p2Bac.His.XPD was a gift from Aziz Sancar (Addgene plasmid # 39217 ; http://n2t.net/addgene:39217 ; RRID:Addgene_39217)