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Addgene

p2Bac.His.XPB
(Plasmid #39216)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2Bac
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7125
  • Total vector size (bp) 9523
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XPB
  • Alt name
    ERCC3
  • Alt name
    excision repair cross-complementing rodent repair deficiency, complementation group 3
  • Alt name
    RAD25
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    ERCC3 (a.k.a. BTF2, GTF2H, RAD25, Ssl2, TFIIH, TTD2, XPB)
  • Promoter Pph
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer polyhedrin_fwd
  • 3′ sequencing primer polyhedrin_rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2Bac.His.XPB was a gift from Aziz Sancar (Addgene plasmid # 39216 ; http://n2t.net/addgene:39216 ; RRID:Addgene_39216)
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