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Addgene

pBA2SkIKmA2
(Plasmid #39214)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    Synthetic Biology
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synechocystis kudzu IspS
  • Alt name
    S-k-IspS
  • Species
    Pueraria montana
  • Insert Size (bp)
    1725
  • GenBank ID
    AY316691
  • Promoter psbA2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XbaI, BamHI (not destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bentley FK, Melis A (2012) Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms. Biotech Bioeng 109:100-109

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA2SkIKmA2 was a gift from Anastasios Melis (Addgene plasmid # 39214 ; http://n2t.net/addgene:39214 ; RRID:Addgene_39214)

  • For your References section:

    Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Lindberg P, Park S, Melis A. Metab Eng. 2010 Jan;12(1):70-9. Epub 2009 Oct 13. 10.1016/j.ymben.2009.10.001 PubMed 19833224
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