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pHAC111-LST8 (pSW75)
(Plasmid #39211)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac111
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LST8
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1700
  • Entrez Gene
    LST8 (a.k.a. YNL006W)
  • Promoter LST8

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13pUC-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses LST8 from LST8 promoter. Cloned as 1.7-kb SacI-PstI fragment into YCplac111. CYC1 terminator (CEN, LEU2)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAC111-LST8 (pSW75) was a gift from Michael Hall (Addgene plasmid # 39211 ; http://n2t.net/addgene:39211 ; RRID:Addgene_39211)

  • For your References section:

    Molecular organization of target of rapamycin complex 2. Wullschleger S, Loewith R, Oppliger W, Hall MN. J Biol Chem. 2005 Sep 2;280(35):30697-704. Epub 2005 Jul 7. 10.1074/jbc.M505553200 PubMed 16002396
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