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Addgene

pCI-HA-mAVO3 (pRL62-3)
(Plasmid #39210)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-HA
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mAVO3
  • Alt name
    Mammalian AVO3
  • Species
    M. musculus (mouse)
  • Mutation
    G5S and I15V
  • Entrez Gene
    Rictor (a.k.a. 4921505C17Rik, 6030405M08Rik, AVO3, D530039E11Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI/BssHII (destroyed during cloning)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCI-HA-based construct (with MluI/BssHII-SmaI sites) that expresses M. musculus mAVO3 N-terminally fused to an HA-epitope.

Addgene's sequencing results found G5S and I15V mutations in the AVO3 sequence when compared to GenBank ID NP_084444.3. This plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-HA-mAVO3 (pRL62-3) was a gift from Michael Hall (Addgene plasmid # 39210 ; http://n2t.net/addgene:39210 ; RRID:Addgene_39210)

  • For your References section:

    Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Jacinto E, Loewith R, Schmidt A, Lin S, Ruegg MA, Hall A, Hall MN. Nat Cell Biol. 2004 Nov;6(11):1122-8. Epub 2004 Oct 3. 10.1038/ncb1183 PubMed 15467718
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