pSEYC68-HA-TOR2 (pAS21)
(Plasmid
#39207)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSEYC68
- Backbone size w/o insert (bp) 7800
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTOR2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)11000
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Entrez GeneTOR2 (a.k.a. YKL203C, DRR2)
- Promoter GAL
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N-terminally HA-tagged TOR2 expressed under control of GAL promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEYC68-HA-TOR2 (pAS21) was a gift from Michael Hall (Addgene plasmid # 39207 ; http://n2t.net/addgene:39207 ; RRID:Addgene_39207) -
For your References section:
HEAT repeats mediate plasma membrane localization of Tor2p in yeast. Kunz J, Schneider U, Howald I, Schmidt A, Hall MN. J Biol Chem. 2000 Nov 24;275(47):37011-20. 10.1074/jbc.M007296200 PubMed 10973982
Map uploaded by the depositor.
