pHIV-Luc-ZsGreen
(Plasmid #39196)

• Depositing Lab: Bryan Welm
• Publication: pHIV Lentiviruses (unpublished)

Backbone

• Vector backbone: pHIV-Zsgreen
• Total vector size (bp): 9312
• Modifications to backbone: This plasmid was generated by cloning Luc2P (firefly luciferase) into the EcoRI and XbaI sites of pHIV-ZsGreen (Addgene Plasmid 18121).
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Firefly Luciferase Luc2P
• Insert Size (bp): 1600
• Promoter: EF1-alpha

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EF1a Fwd Primer

Gene/Insert 2

• Gene/Insert name: ZsGreen
• Insert Size (bp): 700
• Promoter: EF1-alpha

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: MscI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: pBluescriptKS Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Zsgreen is from Clontech, and Luc2P is from Promega.
• Articles Citing this Plasmid:
  • 75 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone plasmid, HIV-Zsgreen, was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. Luciferase (Luc2P) was cloned into the EcoRI and XbaI sites to generate this bicistronic lentivirus that expresses both Luciferase and Zsgreen.