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Purpose(Empty Backbone) SGC Empty backbone for bacterial expression
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39188 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGTvL1-SGC
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Backbone manufacturerSGC
- Backbone size (bp) 6988
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- GST TEV (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenone
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGTvL1-SGC was a gift from Nicola Burgess-Brown (Addgene plasmid # 39188 ; http://n2t.net/addgene:39188 ; RRID:Addgene_39188)
Map uploaded by the depositor.
