ACVR2A
(Plasmid
#39180)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39180 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFB-LIC-Bse
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Backbone manufacturerSGC
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Vector typeBaculovirus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACVR2A
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Alt nameactivin A receptor, type II
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Alt namePDB:3SOC
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SpeciesH. sapiens (human)
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Insert Size (bp)1018
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Entrez GeneACVR2A (a.k.a. ACTRII, ACVR2)
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Tag
/ Fusion Protein
- His Tev (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Fbac-1
- 3′ sequencing primer Fbac-2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ACVR2A was a gift from Nicola Burgess-Brown (Addgene plasmid # 39180 ; http://n2t.net/addgene:39180 ; RRID:Addgene_39180)