UXS1
(Plasmid #39162)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC+year (unpublished)

Backbone

• Vector backbone: pLIC-SGC1
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: UXS1
• Alt name: UDP-glucuronate decarboxylase 1 [Genbank entry]
• Alt name: PDB:2B69
• Species: H. sapiens (human)
• Insert Size (bp): 1042
• Mutation: codon-optimized
• Entrez Gene: UXS1 (a.k.a. SDR6E1, UGD, hUXS, hUXS1)
• Tag / Fusion Protein:
  • His Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/2B69/