SPSB1
(Plasmid #38981)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC+year (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SPSB1
• Alt name: SPRY domain-containing SOCS box protein SSB-1
• Alt name: PDB:2JK9
• Species: H. sapiens (human)
• Insert Size (bp): 717
• Mutation: Residues 24-233 of SPSB1 are present in this plasmid
• Entrez Gene: SPSB1 (a.k.a. SSB-1, SSB1)
• Tag / Fusion Protein:
  • 6xHis-Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/2JK9/