PTPN11
(Plasmid #38965)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Nicola Burgess-Brown
• Publication: SGC+year (unpublished)

Backbone

• Vector backbone: pNIC28-Bsa4
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PTPN11
• Alt name: protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1)
• Alt name: PDB:3B7O
• Species: H. sapiens (human)
• Insert Size (bp): 976
• GenBank ID: protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1)
• Entrez Gene: PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
• Tag / Fusion Protein:
  • His Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

http://www.thesgc.org/structures/3B7O/