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K-p31
(Plasmid #38790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR4.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3956
  • Total vector size (bp) 4788
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3742-4573 of MWPyV
  • Alt name
    Partial LTAg
  • Alt name
    Partial STAg
  • Species
    MW polyomavirus, strain MA095
  • Insert Size (bp)
    832
  • Mutation
    One SNP in comparison to MA095
  • GenBank ID
    JQ898291
  • Promoter lac

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is the positive control plasmid for the MWPyV LTAg qPCR assay described in Siebrasse, et al.

Plasmid contains nucleotides 3742-4573 of GenBank ID JQ898291. The single nucleotide mismatch is at bp# 4365 when compared to this reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K-p31 was a gift from David Wang (Addgene plasmid # 38790 ; http://n2t.net/addgene:38790 ; RRID:Addgene_38790)

  • For your References section:

    Identification of MW polyomavirus, a novel polyomavirus in human stool. Siebrasse EA, Reyes A, Lim ES, Zhao G, Mkakosya RS, Manary MJ, Gordon JI, Wang D. J Virol. 2012 Jun 27. 10.1128/JVI.01210-12 PubMed 22740408
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