K-p22
(Plasmid
#38787)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR4.0
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3956
- Total vector size (bp) 5253
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name2545-3841 of MWPyV
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Alt namePartial LTAg
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SpeciesMW polyomavirus, strain WD976
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Insert Size (bp)1297
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Mutation5 SNPs in comparison to WD976
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GenBank IDJQ898292
- Promoter lac
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains nucleotides 2545-3841 of GenBank ID JQ898292. The five nucleotide mismatches are at bp# 2545-2548 and 3838 when compared to this reference sequence.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K-p22 was a gift from David Wang (Addgene plasmid # 38787 ; http://n2t.net/addgene:38787 ; RRID:Addgene_38787) -
For your References section:
Identification of MW polyomavirus, a novel polyomavirus in human stool. Siebrasse EA, Reyes A, Lim ES, Zhao G, Mkakosya RS, Manary MJ, Gordon JI, Wang D. J Virol. 2012 Jun 27. 10.1128/JVI.01210-12 PubMed 22740408