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Addgene

pCMV-Wnk1
(Plasmid #38780)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38780 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnk1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    6381
  • GenBank ID
    AF227741
  • Entrez Gene
    Wnk1 (a.k.a. Hsn2, Prkwnk1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Wnk1 was a gift from Melanie Cobb (Addgene plasmid # 38780 ; http://n2t.net/addgene:38780 ; RRID:Addgene_38780)

  • For your References section:

    WNK1, a novel mammalian serine/threonine protein kinase lacking the catalytic lysine in subdomain II. Xu B, English JM, Wilsbacher JL, Stippec S, Goldsmith EJ, Cobb MH. J Biol Chem. 2000 Jun 2;275(22):16795-801. 10.1074/jbc.275.22.16795 PubMed 10828064
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