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Addgene

pNestin-EGFP
(Plasmid #38777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nestin 2nd intron enhancer with TK mini promoter
  • Species
    H. sapiens (human)
  • GenBank ID
    NG_012300.1
  • Entrez Gene
    NES (a.k.a. Nbla00170)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNestin-EGFP was a gift from Wei Cui (Addgene plasmid # 38777 ; http://n2t.net/addgene:38777 ; RRID:Addgene_38777)

  • For your References section:

    Generation of human embryonic stem cell reporter lines expressing GFP specifically in neural progenitors. Noisa P, Urrutikoetxea-Uriguen A, Li M, Cui W. Stem Cell Rev. 2010 Sep;6(3):438-49. 10.1007/s12015-010-9159-9 PubMed 20506046
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