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Addgene

myc Rif-TN
(Plasmid #38769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rif
  • Alt name
    Rho in filopodia
  • Alt name
    RHOF
  • Species
    H. sapiens (human)
  • Mutation
    T33N dominant-negative mutation
  • Entrez Gene
    RHOF (a.k.a. ARHF, RIF)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

human Rif GTPase (RHOF) with N-terminal myc-tag in pcDNA3. Dominant-negative mutation of T33N

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc Rif-TN was a gift from Harry Mellor (Addgene plasmid # 38769 ; http://n2t.net/addgene:38769 ; RRID:Addgene_38769)

  • For your References section:

    The Rho family GTPase Rif induces filopodia through mDia2. Pellegrin S, Mellor H. Curr Biol. 2005 Jan 26;15(2):129-33. 10.1016/j.cub.2005.01.011 PubMed 15668168
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