pETM41-EcPPXc
(Plasmid #38329)

• Depositing Lab: Florian Freimoser
• Publication: Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.

Backbone

• Vector backbone: pETM41
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E. coli polyphosphatase
• Alt name: PPX
• Species: Escherichia coli
• Mutation: C-terminus of E. coli polyphosphatase (described as a polyphosphate binding domain) - amino acid 305 (nucleotide 913) until end
• Entrez Gene: ppx (a.k.a. ECO111_3226)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • MBP (N terminal on backbone)
  • TEV (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.