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Addgene

pKM263-EcPPXc
(Plasmid #38328)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKM263
  • Backbone manufacturer
    K. Melcher
  • Total vector size (bp) 5752
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli polyphosphatase
  • Alt name
    PPX
  • Species
    Escherichia coli
  • Mutation
    C-terminus of E. coli polyphosphatase (described as a polyphosphate binding domain) - amino acid 305 (nucleotide 913) until end.
  • Entrez Gene
    ppx (a.k.a. ECO111_3226)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on backbone)
    • GST (N terminal on backbone)
    • TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See "Bolesch, D.G., Keasling, J.D., 2000. Polyphosphate binding and chain length recognition of Escherichia coli exopolyphosphatase. J. Biol. Chem. 275, 33814–33819" for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM263-EcPPXc was a gift from Florian Freimoser (Addgene plasmid # 38328 ; http://n2t.net/addgene:38328 ; RRID:Addgene_38328)

  • For your References section:

    Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry. Werner TP, Amrhein N, Freimoser FM. Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20. 10.1016/j.fgb.2007.01.008 PubMed 17320430
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