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Addgene

pKM263-ScPPX
(Plasmid #38327)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38327 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pKM263
  • Backbone manufacturer
    K. Melcher
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PPX1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PPX1 (a.k.a. YHR201C)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • GST (N terminal on backbone)
    • TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM263-ScPPX was a gift from Florian Freimoser (Addgene plasmid # 38327 ; http://n2t.net/addgene:38327 ; RRID:Addgene_38327)
  • For your References section:

    Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase. Werner TP, Amrhein N, Freimoser FM. Arch Microbiol. 2005 Nov;184(2):129-36. Epub 2005 Oct 21. 10.1007/s00203-005-0031-2 PubMed 16184370