pEF6.mCherry-TSG101
(Plasmid #38318)

• Depositing Lab: Quan Lu
• Publication: Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51. Epub 2012 Feb 6.

Backbone

• Vector backbone: pEF6
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5780
• Total vector size (bp): 7666
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSG101
• Species: H. sapiens (human)
• Insert Size (bp): 1881
• GenBank ID: NM_006292.3
• Entrez Gene: TSG101 (a.k.a. TSG10, VPS23)
• Promoter: EF1
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHRev

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a mutation in the TSG101 ORF, Y82H. This mutation should not affect plasmid function.