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Addgene

pEF6.HA-ARRDC3
(Plasmid #38317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7063
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARRDC3
  • Species
    H. sapiens (human)
  • Entrez Gene
    ARRDC3 (a.k.a. TLIMP)
  • Promoter EF1
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6.HA-ARRDC3 was a gift from Quan Lu (Addgene plasmid # 38317 ; http://n2t.net/addgene:38317 ; RRID:Addgene_38317)

  • For your References section:

    Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to mediate ubiquitination of the beta2-adrenergic receptor. Nabhan JF, Pan H, Lu Q. EMBO Rep. 2010 Aug;11(8):605-11. Epub 2010 Jun 18. 10.1038/embor.2010.80 PubMed 20559325
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