pCI-neo.mCherry-NEDD4
(Plasmid #38316)

• Depositing Lab: Quan Lu
• Publication: Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 2010 Jun 18.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 8855
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nedd4
• Species: H. sapiens (human)
• Insert Size (bp): 3411
• GenBank ID: NM_006154.2
• Entrez Gene: NEDD4 (a.k.a. NEDD4-1, RPF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T3 promoter

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry