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Addgene

pCI-neo.mCherry-NEDD4
(Plasmid #38316)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38316 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 8855
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nedd4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3411
  • GenBank ID
    NM_006154.2
  • Entrez Gene
    NEDD4 (a.k.a. NEDD4-1, RPF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T3 promoter
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo.mCherry-NEDD4 was a gift from Quan Lu (Addgene plasmid # 38316 ; http://n2t.net/addgene:38316 ; RRID:Addgene_38316)
  • For your References section:

    Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to mediate ubiquitination of the beta2-adrenergic receptor. Nabhan JF, Pan H, Lu Q. EMBO Rep. 2010 Aug;11(8):605-11. Epub 2010 Jun 18. 10.1038/embor.2010.80 PubMed 20559325