pGEX-6P-1 Stu2 1-590
(Plasmid #38314)

• Depositing Lab: Anthony Hyman
• Publication: Widlund et al Mol Biol Cell. 2012 Sep 19.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone manufacturer: GE healthcare
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 6748
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 5' 1770 bp from STU2 ORF
• Alt name: STU2 TOG12
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1770
• Entrez Gene: STU2 (a.k.a. YLR045C)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry