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pCM-HA-Gas1
(Plasmid #38313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG416Gal-ccdB
  • Backbone manufacturer
    Addgene 14147
  • Backbone size w/o insert (bp) 5173
  • Total vector size (bp) 8111
  • Modifications to backbone
    The gal promoter was removed from pAG416GalccdB by cutting with SacI (2792) and XbaI (3255, making blunt ends which were filled with Klenow and then ligated.
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gas1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2938
  • Mutation
    contains promoter region
  • Entrez Gene
    GAS1 (a.k.a. YMR307W, CWH52, GGP1)
  • Promoter Endogenous
  • Tag / Fusion Protein
    • 2xHA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The primers for the genomic PCR and the location of the HA tag are from Fujita, MBC, 17,834,2006.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM-HA-Gas1 was a gift from Susan Lindquist (Addgene plasmid # 38313 ; http://n2t.net/addgene:38313 ; RRID:Addgene_38313)

  • For your References section:

    Inhibiting GPI Anchor Biosynthesis in Fungi Stresses the Endoplasmic Reticulum and Enhances Immunogenicity. McLellan CA, Whitesell L, King OD, Lancaster AK, Mazitschek R, Lindquist S. ACS Chem Biol. 2012 Jun 22. 10.1021/cb300235m PubMed 22724584
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