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Addgene

pAT206/Sept7shRNA
(Plasmid #38299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSilencer 2.0-U6
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 3130
  • Total vector size (bp) 3183
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Septin 7
  • Alt name
    sept7KD2
  • Alt name
    Cdc10
  • Alt name
    E430034N22
  • gRNA/shRNA sequence
    GGATCCGTTTGACCAATTT
  • Species
    M. musculus (mouse)
  • GenBank ID
    AK078895 NC_000075.6
  • Entrez Gene
    Septin7 (a.k.a. Cdc10, E430034N22, Sept7)
  • Promoter Human U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer LKO1-5
  • 3′ sequencing primer M13pUC-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT206/Sept7shRNA was a gift from Matthew Krummel (Addgene plasmid # 38299 ; http://n2t.net/addgene:38299 ; RRID:Addgene_38299)

  • For your References section:

    Amoeboid T lymphocytes require the septin cytoskeleton for cortical integrity and persistent motility. Tooley AJ, Gilden J, Jacobelli J, Beemiller P, Trimble WS, Kinoshita M, Krummel MF. Nat Cell Biol. 2009 Jan;11(1):17-26. Epub 2008 Nov 30. 10.1038/ncb1808 PubMed 19043408
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